Detection kit for His-tagged proteins on Western blots: contains Ni-NTA-Gold and metallographic amplification reagent. GoldiBlot? His Western Blot Kit is intended and optimized for direct visualization of recombinant His-tagged proteins and other proteins bearing different histidine tags in western or dot blotting applications. Unlike detection by anti-6xHis antibodies, GoldiBlot? His detection does not require a specific location of the polyhistidine tag (N- or C-terminus), does not need primary and secondary antibody incubations, or the presence of specific adjacent amino acid sequences.