Western Blot (WB), Immunofluorescence Microscopy (IF), IHC, IP
Reviews / Ratings
If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
Purified anti-O-GlcNAc. The monoclonal antibody RL2 reacts specifically with beta-O-linked N-acetylglucosamine (O-GlcNAc). It recognizes o-GlcNAc in beta O-glycosidic linkage to both serine and threonine. However, it exhibits no cross reactivity with alpha-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin. This antibody readily detects in vivo changes in O-GlcNAc modification1.O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all metazoans2. O-GlcNAcylation is similar to protein phosphorylation. Research on O-GlcNAc is particularly relevant to chronic human diseases such as diabetes, cardiovascualr disease, neurodegenerative disorders, and cancer3.Like phosphorylation, the specific functions of O-GlcNAc depend on the protein and sites to which the glycan is attached2. One of the major functions of O-GlcNAc is to prevent O-phosphorylation and to modulate signaling and transcription in response to cellular nutrients or stress4.
Immunogen
O-GlcNAc in beta-O-glycosidic linkage to both serine and threonine.