ELISA, Immunohistochemistry, Western Blot. This Protein G purified antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Approximately 0.7 μg of antibody is sufficient to detect 5 μg of SIN-1 treated BSA by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110).
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Description
This Protein G purified monoclonal antibody reacts with proteins containing 3-nitrotyrosine moieties and does not detect non-nitrated tyrosine residues. This antibody is known to react with nitrated tyrosine residues from human, mouse, rat and dog sources. Reactivity with nitrotyrosine from other sources is also expected.
Immunogen
This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid - BSA conjugate.