Primary Human Venous Endothelial Cells are pooled primary isolates from 250 individual human Donor umbilical veins.These cells were originated using Complete Serum-Free Medium Kit With AcceSup? are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.These cells are available in both cryopreserved vials as well as in 25cm2 and 75cm2 proliferating culture flasks.Each vial or flask of cells is shipped to Customer with Bac-O (antibiotic) and AcceSup?(animal derived growth factors) or AcceSup-R?(human recombinant growth factors) at no additional cost.