Mouse Bone Marrow Neutrophils are derived from the tibias and femurs of pathogen-free laboratory adult mice. Cells are immediately cryo-preserved after isolation. Each vial contains at least 10 x10^6 cells and is delivered at room temperature. Mouse Bone Marrow Neutrophils are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be used for designed experiments. Mouse Bone Marrow Neutrophils were tested for expression of markers using antibodies, CD11b by flow cytometry. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.