Mouse Brain Vascular Smooth Muscle Cells from AcceGen Biotech are isolated from tissue of pathogen-free laboratory mice. Mouse Brain Vascular Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Biotech's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Brain Vascular Smooth Muscle Cells are characterized by immunofluorescent cell staining with antibodies of α-smooth muscle actin and are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen Biotech.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.