Purity: > 85% by SDS - PAGE Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 100 mM NaCl, 0.1 mM PMSF ,10% Glycerol . DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I. Recombinant human DR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography
Size
0.5mg, 100ug, 20ug
Concentration
n/a
Applications
SDS-PAGE
Other Names
Down-regulator of transcription 1 TBP-binding, NC2, NC2-BETA, Down-regulator of transcription 1, TBP-binding