DuSP10, 149-482aa, Human, His tag, E.coli (Bioactivity Validated)
Description
Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200mM NaCl. DuSP10 (Dual specificity protein phosphatase 10) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases(DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. DuSP10 has been shown to interact with MAPK14 and MAPK8. When expressed in mammalian cells, DuSP10 blocks the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK. Recombinant human DuSP10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Size
0.5mg, 100ug, 20ug
Concentration
n/a
Applications
Enzyme activity
Other Names
Dual specificity protein phosphatase 10, MKP-5, MKP5