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Description
We have generated a mAb that identifies human pDCs ex vivo. In blood, 211H6 antigen is highly expressed on IL3Ralpha+ BDCA-2+ CD11c- pDCs. It does not stain lymphocytes, monocytes, basophils and granulocytes. In vitro it is also expressed on monocyte-derived DCs. Upon maturtaion in IL-3 and CD40-L, or with influenza virus, 211H6 is maintained at the cell surface. In situ 211H6 recognizes some isolated cells in T cell zones of tonsils, lymphatic vessels and high endothelial venules. THe 211H6 antigen has been identified as BDCA-4. Neuropilin-1 is a membrane-bound co-receptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin (SEMA3A) family members. Neuropilin 1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration and invasion. (Dzionek A and al., 2000 J. Immunol., 165: 6037-6046)