Primary Human Liver Sinusoidal Microvascular Endothelial Cells were initiated from normal human liver tissue by elutriation following dispase treatment of tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup? are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.