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| Antigenic Specificity | Sars Nucleocapsid Protein |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | sars |
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| Isotype | n/a |
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| Format | Protein A purified |
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| Size | 500 μg |
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| Concentration | 5.0 mg/mL |
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| Applications | ELISA, IF Microscopy, Western Blot. This protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46 kDa in size corresponding to SARS Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein. The product was purified from monospecific antiserum by protein A affinity purification. BLAST analysis was used to suggest reactivity with related Coronavirus proteins. Cross reactivity with homologues from other sources has not been determined. |
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| Immunogen | This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus Nucleocapsid protein. Lifesensors Inc. (www.lifesensors.com) prepared the Nucleocapsid protein as follows: SUMO-Nucleocapsid fusion was expressed in E.coli in LB medium and purified using using Ni-NTA resin (Qiagen) affinity chromatography. After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the nucleocapsid using MAC and the nucleocapsid was finally purified using Cation Exchange Chromatography with the Macro-Prep High S resin (BioRad) and size exclusion chromatography. |
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| Other Names | rabbit anti-Sars Nucleocapsid Protein Antibody, rabbit anti-Sars-CoV Nucleocapsid (N) Protein Antibody, N antibody, N structural protein antibody, NC antibody, Nucleocapsid protein antibody, Nucleoprotein antibody, SARS coronavirus N protein antibody, SARS CoV antibody, SARSCoV antibody, Severe acute respiratory syndrome antibody |
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| Gene, Accession # | N, Gene ID: 1489678, NCBI: 30173007, UniProt: P59595 |
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| Catalog # | 200-401-A50 |
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| Price | |
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| Order / More Info | Sars Nucleocapsid Protein Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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