Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen. Optimal antibody dilution should be determined by titration; recommended range is 1:50 - 1:100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
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Description
The Integrin beta 1D Antibody (1G2) from Novus Biologicals is a mouse monoclonal antibody to Integrin beta 1D. This antibody reacts with human, mouse. The Integrin beta 1D Antibody (1G2) has been validated for the following applications: Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen. Specificity: 1G2 recognizes specifically the cytoplasmic domain of integrin subunit beta 1D present in cardiac and skeletal muscle
Immunogen
Clone 1G2 is a mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to the Cterminal 24 amino acids of integrin beta1D including an appending N-terminal cysteine (CQENPIYKSPINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.