human, mouse, rat, porcine, bovine, Drosophila, insect, primate
Isotype
n/a
Format
immunogen affinity purified
Size
0.025 ml
Concentration
1.0 mg/ml
Applications
Western Blot, Simple Western, Chromatin Immunoprecipitation, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin. In Western Blot, a band is seen 19.5 kDa representing COX IV. In ICC/IF, mitochondrion staining was observed in HeLa cells. In IHC-P, staining is observed in the cytoplasm and mitochondria of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Higher dilutions may be needed for mitochondrial membrane enriched preparations. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
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Description
The COX4 Antibody from Novus Biologicals is a rabbit polyclonal antibody to COX4. This antibody reacts with human, mouse, rat, porcine, bovine, drosophila, insect, primate. The COX4 Antibody has been validated for the following applications: Western Blot, Simple Western, Chromatin Immunoprecipitation, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
Immunogen
A synthetic peptide made to an internal region of human COX IV isoform 1 (within residues 1-100). [Swiss-Prot# P13073]
Other Names
COX IV-1, COX4-1, COX4COXIV, Cytochrome c oxidase polypeptide IV, cytochrome c oxidase subunit 4 isoform 1, mitochondrial, cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV, MGC72016