Fatty Acid Transport Protein 1 (FATP1) BioAssay? ELISA Kit (Rat)
Description
Fatty Acid Transport Protein 1 (FATP1) BioAssay? ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Transport Protein 1 (FATP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Transport Protein 1 (FATP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Transport Protein 1 (FATP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fatty Acid Transport Protein 1 (FATP1) in the samples is then determined by comparing the O.D. of