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Description
The antibody was affinity purified using phospho-ERα (Tyr-537) peptide (without carrier). This antibody detects several forms of ERα ranging from 66 to 35 kDa on SDS-PAGE immunoblots of MCF-7 cells treated with pervanadate, and this reactivity is removed after alkaline phosphatase treatment.
Immunogen
Clone M545 was generated from a phospho-ERα (Tyr-537) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Tyr-537 in human ERα. This sequence is well conserved in rat and mouse ERα, and is also well conserved in ERβ (Tyr-488).