Mouse Resident Peritoneal Macrophages are derived from the peritoneal cavity of pathogen-free laboratory adult mice. Each vial contains at least 8 x10^6 cells and is delivered frozen. Mouse Resident Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture platetandard biochemical procedures performed with cell ready for experiments under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended. Mouse Resident Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.