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Description
The GHRF (Growth Hormone Releasing Factor) Antibody was raised to rat hypothalamic GHRF (1-43). The antibody produces strong labeling of GHRH at dilutions of 1/200-1/400 using indirect immunofluorescence and at dilutions of 1/2,000 - 1/4,000 using biotin/streptavidin HRP in rat hypothalamus (median eminence). Cross reactivity of GHRF antiserum was examined using the paper spot technique of Larsson (1981). Using 2 μL, 100 pmole amounts, the following substances did not react with rat GHRF antisera diluted 1/500 using the PAP labeling method: glucagon, gastric inhibitory peptide, secretin, vasoactive intestinal peptide, peptide histidine isoleucine, pancreatic polypeptide (human or rat), human GHRF, somatostatin, insulin, ACTH, motilin, cholecystokinin octapeptide, substance P, molluscan cardioexcitatiory peptide, gastrin 34, and serotonin. GHRF antiserum had a very good reactivity using rat GHRF at 2 μL, 100 pmole amounts.