Phospholipase D (PLD) Activity Colorimetric Assay Kit
Description
Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimers disease, stroke, cancer and other brain disorders. Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline fromPhosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples.
Size
100 assays
Concentration
n/a
Applications
Measurement of PLD activity in various tissues/cells Analyzing intracellular signaling Screening of PLD inhibitors