Pyruvate Dehydrogenase (PDH) (EC 1.2.4.1) has a vital role in carbohydrate metabolism. It forms a well-characterized enzyme complex with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). PDH converts pyruvate into acetyl-CoA in the presence of NAD and CoA, and links glycolysis to the citric acid cycle. PDH activity is inhibited by high intracellular ratios of ATP/ADP, NADH/NAD or Acetyl-CoA/CoA. In humans, PDH deficiency reduces mitochondrial function and is linked to neurodegenerative diseases. PDH deficiency is X-linked; it results in 2 forms of abnormality: a metabolic form (lactic acidosis) and a neurological form (seizure and/or neuropathological spasm). Recent studies show that PDH is a target of oncogene-induced senescence; activation of PDH enhances pyruvate utilization and increases respiration and redox stress. PDH assay kit provides a quick and easy way for monitoring PDH activity in various samples. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Size
100 assays
Concentration
n/a
Applications
Measurement of pyruvate dehydrogenase activity in various tissues/cellsAnalysis of cell signaling pathway