Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
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Description
Epitope: Cytoplasmic domain; aa 1236-1302 of isoform B of Uniprot ID P20241. Differential splicing generates a nervous system-specific form of neuroglian. The antibody recognizes a specific form of neuroglian via a cytoplasmic domain generated by alternative splicing. Staining is first seen at about 6 hrs in a subset of neurons, but rapidly expands to include all neurons of the CNS and PNS. It is an exceptionally useful marker for the neurons of the neuron pattern in the PNS and developing eye disc. The PNS staining includes a small number of non-neuronal support cells such as the innermost sheath cell associated with the chordotonal neurons. This antibody works well on Westerns. BP 104 requires detergent permeabilization of tissue to bind to the protein, suggesting that it recognizes an epitope specific to the cytoplasmic domain of this form (isoform B) of the protein [PMID 1693086].
Immunogen
Membrane protein preparation from cultured embryonic Drosophila neurons: Total protein