Immunofluorescence, Immunohistochemistry, Western Blot
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Description
Epitope: Amino-terminal cytoplasmic domain; aa 1-280. Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis. The antibody stains restricted regions during the blastoderm stage and intensely stains cells at the ventral furrow. Expression expands to include almost all cells by the end of gastrulation, but later becomes restricted to, and very strongly expressed in, the developing nervous system. It is especially useful for looking at the pattern of neuroblasts. The antibody cannot be used to stain dissections without detergent. The antibody works well on Westerns, but not well for immunoprecipitations or affinity purifications. The corresponding gene has been isolated by expression cloning.
Immunogen
Membrane proteins from cultured embryonic Drosophila neuronal cells: Total protein