Recombinant Human Poly (A)-specific ribonuclease PARN (PARN)
Description
Purity > 90% as determined by SDS-PAGE. MW: 77.5kDa. Full Length: 1-639aa. N-terminal 6xHis-tagged. Homo sapiens (Human). 3'-exoribonuclease that has a preference for poly (A) tails of mRNAs, thereby efficiently degrading poly (A) tails. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early bryonic development. Interacts with both the 3'-end poly (A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly (A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain prature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elents (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the roval of poly (A) tails of AREs mRNAs, which constitutes the first step of destabilization.