Protein Disulfide Isomerase A2 (PDIA2) BioAssay? ECL ELISA Kit (Human)
Description
Protein Disulfide Isomerase A2 (PDIA2) BioAssay? ECL Kit (Human) is a quantitative, in vitro, Chemiluminescence-based sandwich immunoassay (ECL) suitable for use in Tissue homogenates and other biological fluids. Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protein Disulfide Isomerase A2 (PDIA2) level in the sample or standard. Detection Range: 0.156-10ng/ml Sensitivity: The minimum detectable dose of this kit is typically less than 0.051ng/ml. Specificity: This sa