Acetoacetate (AcAc), a beta-ketoacid, is one of the three ketone bodies and is formed via condensation of two molecules of acetyl-CoA in liver mitochondria. AcAc can be enzymatically reduced to 3-beta-hydroxybutyrate (beta-HB), or decarboxylated producing acetone (CH3)2CO). Ketone bodies (betaHB: 78%; AcAc: 20% & CH3)2CO: 2%) are mainly used as an alternative energy source when glucose cannot be delivered to the system. Excessive concentration of ketone bodies (ketoacidosis) is observed in patients with Type I diabetes, severe starvation or alcoholism. Traditionally, AcAc levels have been qualitatively detected using dipsticks that use sodium nitroferricyanide as a chromophore. LSBio's Acetoacetate Assay Kit has adapted that principle with a modification that provides a sensitive method to quantitate endogenous levels of AcAc in human blood, and urine. In this non-enzymatic assay, AcAc reacts with a substrate to generate a colored product that can be measured at 550 nm. The reaction is specific for AcAc and does not detect 3-beta-hydroxybutyrate. The assay kit can detect samples containing acetoacetate as low as 25 μM. . Sample types: biological fluids, blood, plasma, serum, urine
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This assay provides for the detection and measurement of Acetoacetate (AcAc) in various sample types, and analysis of acetoacetate in pathological conditions such as Type I diabetes and alcoholism. Specific for acetoacetate and does not detect 3-Beta-hydroxybutyrate.