Purity >98%. 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2 O2 . This colorless, non-fluorescent reagent reacts with H2 O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2 O2 at a concentration as low as 5 pmol per 100 μl sample.
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Other Names
1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-ethanone, A 6550, ADHP, Amplex Red?