Galactokinase, Recombinant, Human (Galactokinase 1, GALK1, Galactose Kinase)
Description
Purity ~90%. Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts in the adult population. GALK1 sequence shares the greatest level of conservation, 44.5% identity with that from E. coli and 34.6% amino acid identity with the product of the human GALK2 gene. Source: Recombinant human galactokinase, full length, with N-terminal His tag, expressed in E. coli (NM_000154). Specific Activity: ~200pmoles/min/ug Assay Conditions: A 50ul Galactokinase reaction is conducted in buffer containing 20mM Tris, pH 7.4, 5mM MgCl2, 50mM sodium chloride, 5uM ATP, 1mM DTT and 1mM substrate D-Galactose at RT for 1 hour. ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.