Bulk OEM Reagents (Reagent 1/ Reagent 2) for automated chemistry analyzers. Cotinine is a nicotine metabolite with half-life of 24 hours in a human body, it is detectable for several days after cessation of tobacco exposure. Screening of patient samples necessitates an accurate and reliable means for the measurement of cotinine concentration. A high throughput detection method with high sensitivity and specificity is presented herein. Cotinine Assay provides a convenient and efficient method to assess cotinine concentration in human urine.