Quantitative colorimetric assay. This assay has high sensitivity and excellent specificity for detection of rat TRPV4. No significant cross-reactivity or interference between rat TRPV4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between rat TRPV4 and all the analogues, therefore, cross reaction may still exist. Sample types: cell lysates, plasma, serum, tissue homogenates. Range: 23.5 - 1500 pg/ml. Precision: Intra-Assay: CV<8% / Inter-Assay: CV<10%. Time: 4.5 hours