This mouse Cathepsin B BioAssay? ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. Sample Type: Serum and plasma (heparin, EDTA), cell culture supernatants. Intended Use: For use in quantitative protein detection, establishing normal range etc. Sensitivity: <5pg/ml Range: 156-10,000pg/ml Specificity: Recognizes natural and recombinant mouse Cathepsin B. This ELISA kit shows no cross-reactivity with other relevant proteins. Test Principle: This assay employs a monoclonal antibody form rat specific for Cathepsin B coated on a 96-well plate. Standards (NSO) and test samples are added to the wells and Cathepsin B present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed wit