This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with dog CIC. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for dog CIC. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of dog CIC in samples. The color development is stopped and the intensity of the col