|This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human pSmad3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in
[protein phosphatase 1A; Protein phosphatase 1A; protein phosphatase 1A; IA; PP2C-alpha; protein phosphatase IA; protein phosphatase 2C isoform alpha; protein phosphatase 1A, magnesium dependent, alpha isoform; Protein phosphatase type 1A (formely 2C) Mg-dependent alpha isoform; Protein phosphatase type 1A (formely 2C), Mg-dependent, alpha isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA]