human, mouse, rat, canine, equine, monkey, primate
Isotype
IgG1 kappa
Format
Protein G purified
Size
0.1 mg
Concentration
1 mg/ml
Applications
Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Functional, In vitro assay, Flow (Intracellular), CyTOF-ready, Block/Neutralize. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a 120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Functional and in vitro assays reported in scientific literature (PMID 25411258). Use in ELISA reported in multiple publications. Use in Immunoprecipitation reported in scientific literature (PMID 25871979). Use in Flow-intracellular reported in scientific literature (PMID 24986635). Use in functional reported in scientific literature (PMID: 25411258). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry reported in scientific literature (PMID 27744078). Use in blocking/ neutralizing reported in scientific literature (PMID: 25338738).
Reviews / Ratings
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Description
The TLR9 Antibody (26C593.2) from Novus Biologicals is a mouse monoclonal antibody to TLR9. This antibody reacts with human, mouse, rat, canine, equine, monkey, primate. The TLR9 Antibody (26C593.2) has been validated for the following applications: Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Functional, In vitro assay, Flow (Intracellular), CyTOF-ready, Block/Neutralize.
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).