The Mouse CD5L ELISA Pair Set is for the quantitative determinationof Mouse CD5L.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD5L coated on a 96-well plate. Standards and samples are added to the wells, and any CD5L present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD5L polyclonal antibody is then added, producing an antibody-antigen-antibody sandwich. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD5L present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.