Recombinant full-length human EIF4E was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
Size
50 μg
Concentration
0.2 μg/μL
Applications
Western Blot. EIF4E Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. EIF4E Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 51kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.