Recombinant full-length human BID was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
Size
50 μg
Concentration
0.2 μg/μL
Applications
Western Blot. BID Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. BID Protein is suitable for use in Western Blot. Expect a band approximately ~ 52kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Other Names
FP497, MGC42355, MGC15319, BH3-interacting domain death agonist, p22 BID, BH3-interacting domain death agonist p15, p15 BID, p13 BID, p11 BID