Mouse IgG Specific Peroxidase Steptavidin-Biotin Complex Immunohistochemistry Detection Kit is a streptavidin-biotin immuno-enzymatic antigen detection system. This system involves the sequential incubation of the sample with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, enzyme-labeled streptavidin, and chromogen substrate. Mouse Specific HRP/DAB (ABC) Detection Kit includes reagents specifically designed for the use in the analysis of antigens in IHC and other immunodetection assays. The provided protocol includes steps for immunohistochemical analysis with low background using 5% BSA blocking reagent, biotinylated secondary antibody, and an enzyme-labeled streptavadin.
Size
1 kit
Concentration
n/a
Applications
Immunohistochemistry. This kit is ready to use and no dilution is needed. Useful for Immunohistochemistry. IHC(P): 1:50-200, IHC(F): 1:50-200, ICC: 1:50-200. Optimal dilutions should be determined by end users.