Western Blotting (WB),ELISA,Immunofluorescence (IF)
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Description
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Immunogen
Glycerol dehydrogenase isolated and purified from Enterobacter aerogenes. Freund's complete adjuvant is used in the first step of the immunization procedure.