Plasminogen Activator Inhibitor Type 1, 6X His-Tag, Human (PAI-1)
Description
Purity ≥99% pure by SDS PAGE 98 ±2% active by uPA titration. Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation (1). The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer (2,3,4). PAI-1 is activated by the glycoprotein vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration (5). This binding has proven to be an important inhibitor of the activated form of protein C (APC) (6). This Human PAI-1 is a collection of four mutations: K154T, Q139L, M354I and H150H. These mutations offer great stability while locking into the active conformaton. The N-terminus has been additionally modified with the coupling of a 6-X His tag. Solubility: >2mg/ml and <5mg/ml Absorbance (280nm): 1.65 E0.1% = 1.0 Kinetic Data: Second order rate constants for