ELISA, Immunohistochemistry, Western Blot. This secondary antibody anti-Dog is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Dog IgG, Dog IgG F(c) and Dog Serum. No reaction was observed against Dog IgG F(ab).
Immunogen
Anti-Dog IgG F(c) whole molecule was produced by repeated immunization with Dog IgG F(c) whole molecule in goat.
Other Names
goat anti-Dog IgG F(c) Antibody Peroxidase conjugation, goat anti-Dog IgG Fc fragment HRP conjugated Antibody, Dog Secondary Antibody