Amplite? Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit. Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway. Animals, fungi, and plants use G3P to produce ATP. It is used to regenerate NAD+ in brain and skeletal muscle cells. G3P has been linked to lipid imbalance diseases such as obesity. Amplite? G3P Assay Kit provides one of the most sensitive methods for quantifying G3P. The kit uses Amplite? Red substrate to quantify the concentration of G3P, which is proportional to the concentration of hydrogen peroxide formed in the enzyme coupling reaction cycle. The kit is an optimized mix and read format that is compatible with HTS applications. It detects as little as 12.5 ?M G3P in 100 ?L assay volume as shown in Figure 1. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read at ~576 +/- 5 nm with an absorbance microplate reader.