Purity 214nm: ~98%, 280nm: ~95% (HPLC). Protein A, Recombinant. Expressed in soy-based media, free of animal proteins. Source: Recombinant protein corresponding to Protein A, expressed in E. coli. Bioburden: No organisms detected. Endotoxin: ≤0.5EU/mg Protein Concentration (OD275): ~50mg/ml 10% SDS-PAGE (Coomassie Stain): Single major band 45kD±1kD hIgG Binding: ≥95% Conductivity: 1.2 E0.1% at 270nm = 0.14-0.20 Triton Content, HPLC: <0.015% Isoelectric Focusing: Single band between 4.6 and 5.2 pI markers.