Purity ~97% (SDS-PAGE, HPLC). The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus and C-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Source: Recombinant protein corresponding to Protein A, fused to 6x His-tag at N-terminus and C-terminus, expressed in E. coli. Molecular Weight: ~39.21kD Endotoxin: <0.1ng/ug Specificity: Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from bovine, guinea pig, hamster, equine, porcine, and rabbit. Protein A has little affinit
Size
100mg
Concentration
n/a
Applications
n/a
Other Names
Protein A, Recombinant, His-Tag (Lyophilized) (Protein A beads, Protein A sepharose, Protein A Sepharose Beads)