ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Immunoprecipitation, Western Blot. Anti-BCL-3 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at 47.5 kDa in western blot using appropriate lysates. Positive control used recombinant BCL3 protein, HEK293T overexpressing BCL3 or U87 whole cell lysates at 1μg/mL in WB; and U87MG with PFA at 10μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.
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Description
This affinity purified antibody is directed against human BCL3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.
Immunogen
Anti-BCL3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human BCL3 conjugated to Keyhole Limpet Hemocyanin (KLH).