ELISA, Immunohistochemistry, Western Blot. Anti-beta Actin antibody has been tested for use in ELISA, IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user.
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Description
Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse β Actin protein. Expected to cross-react with a wide range of species due to sequence homology. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.
Immunogen
Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with β-Actin peptide near the N-Terminus followed by hybridoma development.