Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Anti-CD45.1 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
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Description
Allophycocyanin conjugated CD45.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45.1. Cross reactivity is observed with human and Feline. Cross reactivity with CD45.1 from other sources has not been tested. Anti-CD45.1 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.
Immunogen
Anti-CD45.1 Antibody (Monoclonal) was produced by repeated immunizations with CD45.1 antigen.
Willinger T and Flavell, RA. 2012. Proc. Natl. Acad. Sci. 109: 8670 - 8675.Siggs OM, Li X, Xia Y, and Beutler B. 2012. J. Exp. Med. 209:19-27. Wakim LM, Woodward-Davis A, and Bevan MJ. 2010. 107: 17872-17879.