ELISA, Immunoprecipitation, Western Blot. This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1% saponin treatment or other permeabilization method.
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Description
This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
Lukas, J. et al. (1992) Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur. J. Biochem., 207, 169-176.Ye, X.S. et al. (1997) Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2. J. Biol Chem 272(52):33384-93.Palmer, A. et al. (1998) A link between MAP kinase and p34cdc2/cyclin B during oocyte maturation: p90rsk phosphorylates the p34cdc2 inhibitory kinase Myt1. EMBO J. 17, 5037-5047.