ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. This antibody is suitable for immunoblotting, immunohistochemistry immuno-precipitation (as active kinase), and immunoblotting. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. This product is suitable for the detection by immunoblot of human, rat and mouse cdc2. For immunohistochemistry use paraffin embedded tissue.
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Description
This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 34 kDa cdc2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdc2 from other species may also occur.
Immunogen
cdc2 peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).