FLISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. Fluorescent Mouse TrueBlot? Antibody Fluorescein is provided as a lyophilized powder. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot, cat. 18-0217-32 (100μL) will yield enough reagent for 40 blots.Note that there are three key procedural considerations:1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands.2. Immunoprecipitate should be completely reduced.3. Milk should be used as the blocking protein for the immunoblot.
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Description
Fluorescent Mouse TrueBlot? Antibody Fluorescein Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA.
Immunogen
n/a
Other Names
FITC, TrueBlot, FITC TrueBlot ULTRA, Fluorescein TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, Fluorescent TrueBlot, Ms TrueBlot