ELISA, Western Blot. Anti-Fructose-6-Phosphate Kinase antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.
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Description
Anti-Fructose-6-Phosphate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.