ELISA, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to Mre11 by western blotting in the appropriate cell lysate or extract.
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Description
This affinity purified antibody is directed against mouse Mre11 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Mre11 protein from rat (94% homology). Based on protein sequence homology also expect partial reactivity against Mre11 homologues from macaque (88%), human (84%) and dog (82%). This sequence shows less than 75% homology to homologues from bovine, Xenopus, chicken, chimpanzee and zebrafish sources. Reactivity against homologues from other sources is not known.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corres-ponding to amino acids 68-706 of mouse Mre11 protein.
Other Names
rabbit anti-Mre11 antibody, Double-strand break repair protein MRE11a, DNA recombination and repair protein, Meiotic recombination 11 homolog A, Mre11a